Microscopic and Physiological Analysis of Somatic Embryos Under in Vitro Culture in Triticale
DOI:
https://doi.org/10.46291/ICONTECHvol6iss1pp73-80Anahtar Kelimeler:
Somatic embryos, triticale, tissue culture, enzyme activities, in vitro assayÖzet
Somatik embriyogenez, bitki somatik hücrelerinin in vitro koşullar altında somatik embriyolara dönüşen embriyojenik hücreler ürettiği bir gelişim sürecidir. Somatik embriyogenez, bitkilerin in vitro klonal çoğaltılmasında etkili bir yöntemdir. Olgun embriyolar, embriyojenik kallus oluşumu için eksplant kaynağı olarak kullanıldı ve kallus, kompakt, sağlıklı ve olgun embriyoların çoğu indüklenmiş embriyojenik kallus oluşturdu. Somatik embriyolar, gelişimlerinde somatik olmayan emsallerine morfolojik olarak benzer bir özellik göstermiştir. Karşılaştırmalı bir çalışmada üç tritikale çeşidinin 'Tatlıcak, Alper Bey ve Mikham' somatik embriyolarının embriyojenik kallus evrelerindeki toplam protein, DNSA ve H2O2, MDA gibi bazı antioksidan enzimlerin aktiviteleri analiz edilmiştir. Ancak; somatik embriyolar en yüksek düzeyde DNSA, MDA, Test edilen tüm çeşitlerde H2O2 ve toplam protein. Sonuçlarımız, tritikale somatik embriyo olgunlaşma sürecinin in vitro koşullarda iki yıl içinde tamamlandığını gösterdi.
Referanslar
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