Effects of Rumen Protected Choline and Propylene Glycol on Metabolism in Dairy Cows with Low Body Condition
DOI:
https://doi.org/10.5281/zenodo.15095411Keywords:
Dairy Cows, Low Body Condition, Propylene Glycol, Rumen Protected CholineAbstract
Rumen protected choline (RPC), propylene glycol (PG) are used for preventing fatty liver disease and ketosis. The purpose of this study was to determine the effects of RPC, PG on metabolism in dairy cows with low body condition. The study included 32 multiparous Holstein cows (n=32) aged 3-6 years with a body condition score (BCS) of 2 to 2.5. The animals were divided into two groups using the random sampling method. The experimental group (n=16) received 60 g/d of RPC and 250 ml/d of PG by oral administration for one-week prepartum (7±5 days) and another week postpartum. The control group (n=16) received no RPC and PG. Blood samples were examined for calcium, Gamma-glutamyl transferase (GGT), Non-Esterified Fatty Acids (NEFA), β-hydroxybutyric acid (BHBA), urea, and triglycerides. All cows in the study received the same care and food routine. RPC and PG supplementation had a effect on blood concentrations of NEFA, urea, and triglycerides (p<0.05). Therefore, using RPC and PG could reduce the incidence of ketosis and fatty liver disease by significantly lowering blood triglyceride levels. Accordingly, it is thought that an increase in the NEFA and BHBA ratios could lead to fatty liver and ketosis. Statistical analysis revealed that the oral supplementation of RPC and PG to dairy cows in the experimental group had a statistically significant effect on the blood triglyceride, NEFA concentrations. As a result, using RPC and PG in dairy cows could help to lower the risk of ketosis and fatty liver disease since blood triglyceride levels decreased significantly.
References
Arshad, U., A. Husnain, M.B. Poindexter, R. Zimpel, M.C. Perdomo and J.E.P. Santos (2023). Effect of source and amount of rumen-protected choline on hepatic metabolism during induction of fatty liver in dairy cows. J. Dairy Sci. 106(10): 6860–6879. https://doi.org/10.3168/jds.2023-23270
Arshad, U., M.G. Zenobi, C.R. Staples and J.E.P. Santos (2020). Meta-analysis of the effects of supplemental rumen-protected choline during the transition period on performance and health of parous dairy cows. J. Dairy Sci. 103(1): 282–300. https://doi.org/10.3168/jds.2019-16842
Bell, A.W. (1981). Lipid metabolism in liver and selected tissues and in the whole body of ruminant animals. Lipid Metabolism in Ruminant Animals. 363-410.
Bell, A.W. (1995). Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation. J. Anim. Sci. 73(9): 2804-2819. https://doi.org/10.2527/1995.7392804x
Chung, Y.H., N.E. Brown, C.M. Martinez, T.W. Cassidy and G.A. Varga (2009). Effects of rumen-protected choline and dry propylene glycol on feed intake and blood parameters for Holstein dairy cows in early lactation. J. Dairy Sci. 92(6): 2729–2736. https://doi.org/10.3168/jds.2008-1299
Contreras, G.A., N.J. O'Boyle, T.H. Herdt and L.M. Sordillo (2010). Lipomobilization in periparturient dairy cows influences the composition of plasma nonesterified fatty acids and leukocyte phospholipid fatty acids. J. Dairy Sci. 93(6): 2508–2516. https://doi.org/10.3168/jds.2009-2876
De Vries, M.J. and R.F. Veerkamp (2000). Energy balance of dairy cattle in relation to milk production variables and fertility. J. Dairy Sci. 83(1): 62–69. https://doi.org/10.3168/jds.S0022-0302(00)74856-9
Drackley, J. K. (2001). Management of transition cows: Integrating nutrition and stress physiology. In: Proceedings cornell nutrition conference for feed manufacturers, rochester, New York. 148-159 p.
Herdt, T.H. (2000). Ruminant adaptation to negative energy balance: Influences on the etiology of ketosis and fatty liver. Vet. Clin. North. Am. Food Anim. Pract. 16(2): 215-230.
Holdorf, H.T., S.J. Kendall, K.E. Ruh, M.J. Caputo, G.J. Combs, S.J. Henisz, W.E. Brown, T. Bresolin, R.E.P. Ferreira, J.R.R. Dorea and H.M. White (2023). Increasing the prepartum dose of rumen-protected choline: Effects on milk production and metabolism in high-producing Holstein dairy cows. J. Dairy Sci. 106(9): 5988–6004. https://doi.org/10.3168/jds.2022-22905
Huang, B., M.Z. Khan, X. Kou, Y. Chen, H. Liang, Q. Ullah, N. Khan, A. Khan, W. Chai and C. Wang (2023). Enhancing metabolism and milk production performance in periparturient dairy cattle through rumen-protected methionine and choline supplementation. Metabolites. 13(10): 1080. https://doi.org/10.3390/metabo13101080
Humer, E., G. Bruggeman and Q. Zebeli, (2019). A meta-analysis on the impact of the supplementation of rumen-protected choline on the metabolic health and performance of dairy cattle. Animals (Basel). 9(8): 566. https://doi.org/10.3390/ani9080566
Kristensen, N.B. and B.M. Raun (2007). Ruminal and intermediary metabolism of propylene glycol in lactating Holstein cows. J. Dairy Sci. 90(10): 4707–4717. https://doi.org/10.3168/jds.2007-0295
Li, P., X.B. Li, S.X. Fu, C.C. Wu, X.X. Wang, G.J. Yu, M. Long, Z. Wang and G.W. Liu (2012). Alterations of fatty acid β-oxidation capability in the liver of ketotic cows. J. Dairy Sci. 95(4): 1759–1766. https://doi.org/10.3168/jds.2011-4580
Lima, F.S., M.F. Sá Filho, L.F. Greco and J.E.P. Santos (2024). Rumen-protected choline improves metabolism and lactation performance in dairy cows. Animals (Basel). 14(7): 1016. https://doi.org/10.3390/ani14071016
Marques, T.C., H.F. Monteiro, D.B. Melo, W.M. Coelho Jr, S. Salman, L.R. Marques, K.M. Leão, V.S. Machado, P. Menta, D. Dubey, F. Sun, and F.S. Lima (2024). Effect of rumen-protected choline on dairy cow metabolism, immunity, lactation performance, and vaginal discharge microbiome. J. Dairy Sci. 107(5): 2864–2882. https://doi.org/10.3168/jds.2023-23850
McGarry, J.D. and D.W. Foster, (1979). In support of the roles of malonyl-CoA and carnitine acyltransferase I in the regulation of hepatic fatty acid oxidation and ketogenesis. The J. Biol. Chem. 254(17): 8163–8168.
NASEM, (2021). (National Academies of Science, Engineering, and Medicine) Nutrient Requirements of Dairy Cattle, 8th ed., Washington, DC.,US, National Academies Press https://doi.org/10.17226/25806.
Nielsen, N.I. and K.L. Ingvartsen (2004). Propylene glycol for dairy cows: A review of the metabolism of propylene glycol and its effects on physiological parameters, feed intake, milk production and risk of ketosis. Animal Feed Science and Technolog. 115: 191-213.
Ospina, P.A., J.A. McArt, T.R. Overton, T. Stokol and D.V. Nydam (2013). Using nonesterified fatty acids and β-hydroxybutyrate concentrations during the transition period for herd-level monitoring of increased risk of disease and decreased reproductive and milking performance. Vet. Clin. North Am. Food Anim. Pract. 29(2): 387–412. https://doi.org/10.1016/j.cvfa.2013.04.003
Piepenbrink, M.S. and T.R. Overton (2003). Liver metabolism and production of cows fed increasing amounts of rumen-protected choline during the periparturient period. J. Dairy Sci. 86(5): 1722–1733. https://doi.org/10.3168/jds.S0022-0302(03)73758-8
Ruddick J.A. (1972). Toxicology, metabolism, and biochemistry of 1,2-propanediol. Toxicol. Appl. Pharmacol. 21(1): 102–111. https://doi.org/10.1016/0041-008x(72)90032-4
Ster, C., M.C. Loiselle and P. Lacasse (2012). Effect of postcalving serum nonesterified fatty acids concentration on the functionality of bovine immune cells. J. Dairy Sci. 95(2): 708–717. https://doi.org/10.3168/jds.2011-4695
Studer, V.A., R.R. Grummer, S.J. Bertics and C.K. Reynolds (1993). Effect of prepartum propylene glycol administration on periparturient fatty liver in dairy cows. J. Dairy Sci. 76(10): 2931–2939. https://doi.org/10.3168/jds.S0022-0302(93)77633-X
Sun, F., Y. Cao, C. Cai, S. Li, C. Yu and J. Yao (2016). Regulation of nutritional metabolism in transition dairy cows: energy homeostasis and health in response to post-ruminal choline and methionine. PloS One. 11(8): e0160659. https://doi.org/10.1371/journal.pone.0160659
Taghipour, B., H.A. Seifi, M. Mohri, N. Farzaneh and A. Naserian (2010). Variations of energy related biochemical metabolites during periparturition period in fat-tailed Baloochi breed sheep. Iranian Journal of Veterinary Science and Technology. 2(2): 85-92. doi: 10.22067/veterinary.v2i2.8368
Van Soest, B.J., M.N. Pereira, T.F. Duffield, M.A. Steele and T.J. DeVries (2023). Effect of pre- and postpartum supplementation of a pure glycerol product to dairy cows on feed intake, metabolic markers, and milk yield and components. J. Dairy Sci. 106(10): 6798–6815. https://doi.org/10.3168/jds.2022-23173
Winkelman, L.A., T.H. Elsasser and C.K. Reynolds (2008). Limit-feeding a high-energy diet to meet energy requirements in the dry period alters plasma metabolite concentrations but does not affect intake or milk production in early lactation. J. Dairy Sci. 91(3): 1067–1079. https://doi.org/10.3168/jds.2007-0434
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