Isolation of Trichomonas vaginalis and Investigation of Metronidazole Susceptibility in Patients with Vaginal Discharge

Gönenç Çalışkantürk; Fahriye Ekşi; Özge Kömürcü Karuserci

doi:10.5281/zenodo.10636761

Authors

Gönenç Çalışkantürk Department of Medical Microbiology, Gaziantep University, Faculty of Medicine, Gaziantep, Türkiye https://orcid.org/0000-0002-4973-8175
Fahriye Ekşi Department of Medical Microbiology, Gaziantep University, Faculty of Medicine, Gaziantep, Türkiye https://orcid.org/0000-0003-2245-7979
Özge Kömürcü Karuserci Department of Gynecology and Obstetrics, Gaziantep University, Faculty of Medicine, Gaziantep, Türkiye https://orcid.org/0000-0003-3836-2958

DOI:

https://doi.org/10.5281/zenodo.10636761

Keywords:

Trichomonas vaginalis, Metronidazole, microdilution

Abstract

Trichomonas vaginalis is one of the most important vaginitis agent in the world. Metronidazole is the most frequently chosen antimicrobial agent for trichomoniasis treatment. The aim of this study to investigate the in vitro metronidazole susceptibility of T. vaginalis strains isolated from clinical samples.

This study was performed with 130 female patients with vaginal discharge. Informed consent was obtained from each patient included in the study. Samples were taken from the posterior fornix of the cervix with a sterile cotton swab, were placed in trypticase-yeast extract-maltose (TYM) medium and transported to the microbiology laboratory. Firstly, the presence of trophozoites in the sample was determined with direct microscopic investigation between glass slides. If T. vaginalis trophozoites were detected, they were passaged to a new medium and pure T. vaginalis trophozoites were isolated. The metronidazole susceptibility of isolates was investigated with the microdilution method. Isolates with minimum lethal concentration (MLC) above 50 µg/mL were accepted as resistant.

In one of all samples T. vaginalis was detected by direct microscopic investigation. From samples in TYM cultures, three T. vaginalis isolates were identified. For two of the isolated T. vaginalis strains, the MLC value was 1.56 μg/ml, while the other had an MLC value of 3.12 μg/ml. In light of these data, all the three T. vaginalis isolates were identified to be metronidazole susceptible.

It should not be forgotten that T. vaginalis is an important causative agent of vaginitis.

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Isolation of Trichomonas vaginalis and Investigation of Metronidazole Susceptibility in Patients with Vaginal Discharge

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