VIBURNUM OPULUS VE EQUISETUM ARVENSE EKSTRAKTI TİCARİ FORMÜLASYONLARININ ANTİ-SİTOTOKSİK VE ANTİ-GENOTOKSİK AÇIDAN DEĞERLENDİRİLMESİ

Authors

  • Nergis KAYA Canakkale Onsekiz Mart University

DOI:

https://doi.org/10.46291/ICONTECHvol6iss1pp26-32

Keywords:

Gilaburu, Kırkkilit, sitotoksisite, genotoksisite

Abstract

The effects of commercial formulations of Viburnum opulus and Equisetum arvense extract on anti-cytotoxicity and anti-genotoxicity were evaluated at the root tip of Allium cepa L. Distilled water, DMSO, ethyl methane sulfonate (EMS), 5% and 10% doses of V. opulus and E. arvense extracts were applied for 24 and 48 hours. Cytotoxicity and genotoxicity were evaluated by calculating %mitotic index and %chromosomal aberration index, respectively. When the 24h and 48h application of V. opulus and E. arvense extracts were compared with distilled water and DMSO applications; It was determined that these extracts increased the mitotic index and decreased the chromosomal aberration index. It was found that the mitotic index increased and the chromosomal aberration index decreased depending on the increase in the extract dose for 24h and 48h except for V. opulus. Chromosomal aberrations such as C-mitosis, polar shifting, and laggard chromosome were observed in A. cepa root tips meristematic cells by microscope. As a result of this research, it can be stated that commercial formulations of V. opulus and E. arvense extracts are anti-cytotoxic and anti-genotoxic in A. cepa root tip cells when compared with distilled water, DMSO and EMS groups.

 

References

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Rop O, Reznicek V, Valsikova M, Jurikova T, Mlcek J, Kramarova D (2010). Antioxidant properties of European Cranberrybush fruit (Viburnum opulus var. edule). Molecules.15:4467–4477.

Verschaeve L, Van Staden J (2008). Mutagenic and antimutagenic properties of extracts from South African traditional medicinal plants. J. Ethnopharmacol. 119:575–587.

Wan-Ibrahim WI, Sidik K, Kuppusamy UR (2010). A high antioxidant level in edible plants is associated with genotoxic properties. Food Chem. 122:1139–1144.

Winkworth RC. Viburnum phylogeny based on combined molecular data: implications for taxonomy and biogeography. Am J Botany. 2005;92:653–666.

Yen GC, Lin HT, Cheng YH, Lin YJ, Chang SC, Yeh SD, Chan YC, Chung YC, Liao JW (2011). Food safety evaluation of papaya fruits resistant to papaya ring spot virus. J. Food Drug Anal. 19:269–280.

Yuet Ping, K., Darah, I., Yusuf, U. K., Yeng, C., & Sasidharan, S. (2012). Genotoxicity of Euphorbia hirta: an Allium cepa assay. Molecules, 17(7), 7782-7791.

Agência Nacional de Vigilância Sanitária. Resolução RDC nº. 26, de 13 de maio de 2014. Dispõe sobre o registro de medicamentos fitoterápicos e o registro e a notificação de produtos tradicionais fitoterápicos. Diário Oficial da União 14 mai. Seção 1. 2014.

Agnieszka Stępień, A., David Aebisher, D., & Dorota Bartusik-Aebisher, D. (2018). Anticancer properties of Viburnum. European Journal of Clinical and Experimental Medicine, (1), 47-52.

Al-Snafi AE. Medicinal plants with anticancer effects (part 2)- plant based review. Sch Acad J Pharm 2016; 5(5): 175-193.

Al-Snafi, A. E. (2017). The pharmacology of Equisetum arvense-A review. IOSR Journal of Pharmacy, 7(2), 31-42.

Alves AM, Vidal LS, Kuster RM, Lage C, Leitao AC (2009). Genotoxic and mutagenic effects of Melissa officinalis (ErvaCidreira) extracts. Open Toxicol. J. 3:58–69.

Asgarpanah J, Roohi E. Phytochemistry and pharmacological properties of Equisetum arvense L. J Med Plant Res. 2012;6(21):3689-93.

Beikircher B, Mayr S. Intraspecific differences in drought tolerance and acclimation in hydraulics of Ligustrum vulgare and Viburnum lantana. Tree Physol. 2009;29(6):765-75. 3.

Ceylan D, Aksoy A, Ertekin T, Yay AH, Nisari M, Karatoprak GS, et al. The effects of gilaburu (Viburnum opulus) juice on experimentally induced Ehrlich ascites tumor in mice. J Cancer Res Ther 2018;14:314–20.

Debnath SC, Siow YL, Petkau J, An D, Bykova NV (2012). Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis. Can. J. Plant. Sci. 92:1121–1133.

European commission, A pilot project: proposal for approbation of basic substances, in the context of regulation (EC) N 1107/2009 Equisetum arvense. http://www. itab. asso.fr/downloads/comintrants/4096_dar-4p_rapport-technique _ final_annexes. Pdf

European Medicines Agency. Assessment report on Equisetum arvense L. herb. [updated 2016 Feb 2; cited 2019 Jan 10]. 2016;1-36. Available from: https:// www.ema.europa.eu/en/documents/herbal-report/final-assessment-reportequisetum-arvense-l-herba_en.pdf

Harutyunyan, K., Balayan, K., Tadevosyan, G., Hayrapetyan, M., Musayelyan, R., Grigoryan, R., Babayan, N. (2019). Genotoxic potential of selected medicinal plant extracts in human whole blood cultures. Journal of Herbmed Pharmacology, 8(2), 160-162.

Kalyoncu IH, Ersoy N, Elidemir AY, Karali ME (2013). Some physicochemical characteristics and mineral contents of gilaburu (Viburnum opulus L.) fruits in Turkey. Int. J. Biol. Vet. Agric. Food Eng. 7(6):176– 178.

Karimova AR, Yunusova SG, Maslennikov SI, Galkin EG, Yunusov TS, Shereshovets VV, Yunusov MS (2000). Lipids, lipophilic components and biologically active fractions of Viburnum opulus L. seeds. Chem. Nat. Compd. 36(6):560–564.

Kikuchi M, Onoguchi R, Yaoita Y, Machida K (2011).Two new glycosides from Viburnum plicatumThunb.ex Murray var. plicatum f. plicatum. J. Nat. Med. 65:202–205.

Kollmann J, Grubb P. Viburnum lantana L. and Viburnum opulus L. (V.lobatum lam., Opulus vulgaris Borh.). J Ecol. 2002;90:1044-1070.

Kour, J., Ali, M. N., Ganaie, H. A., & Tabassum, N. (2017). Amelioration of the cyclophosphamide induced genotoxic damage in mice by the ethanolic extract of Equisetum arvense. Toxicology reports, 4, 226-233.

Kraujalytė V, Leitner E, Venskutonis PR (2012). Chemical and sensory characterisation of aroma of Viburnum opulusfruits by solid phase microextraction-gas chromatography–olfactometry. Food Chem.132:717–723.

Kraujalytė V, Venskutonis PR, Pukalskas A, Česonienė L, Daubaras R (2013). Antioxidant properties and polyphenolic compositions of fruits from different European cranberrybush (Viburnum opulusL.) genotypes. Food Chem. 141:3695–3702.

Mennen LI, Walker R, Bennetau-Pelissero C, Scalbert A (2005). Risks and safety of polyphenol consumption. Am. J. Clin. Nutr. 81:326–329.

Ovodova RG, Golovchenko VV, Popov SV, Shashkov AS, Ovodov YS (2000). The isolation, preliminary structural studies, and physiological activity of water-soluble polysaccharides from the squeezed berries of the snowball tree Viburnum opulus. Russ. J. Bioorg. Chem. 26(1):54–59.

Pal M, Singh V, Agrawal J, Tewari SK, Zhao QS (2013). Chemical composition and antimicrobial activity of essential oil of Viburnum betulifolium. J. Med. Sci. 13(1):72–75.

Paulauskas A, Žukauskienė J, Žiaukienė D, Česonienė L, Viškelis P. Differentiation of Viburnum accessions according to their molecular, biochemical, genotoxic and microbiological features of importance to selection. Acad J Agr Res. 2015;3(6):081-093.

Paulauskas, A., Žukauskienė, J., Žiaukienė, D., Česonienė, L., Daubaras, R., Kupčinskienė, E., & Viškelis, P. (2015). Differentiation of Viburnum accessions according to their molecular, biochemical, genotoxic and microbiological features of importance to selection. Academia Journal of Agricultural Research, 3(6), 81-93.

Rop O, Reznicek V, Valsikova M, Jurikova T, Mlcek J, Kramarova D (2010). Antioxidant properties of European Cranberrybush fruit (Viburnum opulus var. edule). Molecules.15:4467–4477.

Verschaeve L, Van Staden J (2008). Mutagenic and antimutagenic properties of extracts from South African traditional medicinal plants. J. Ethnopharmacol. 119:575–587.

Wan-Ibrahim WI, Sidik K, Kuppusamy UR (2010). A high antioxidant level in edible plants is associated with genotoxic properties. Food Chem. 122:1139–1144.

Winkworth RC. Viburnum phylogeny based on combined molecular data: implications for taxonomy and biogeography. Am J Botany. 2005;92:653–666.

Yen GC, Lin HT, Cheng YH, Lin YJ, Chang SC, Yeh SD, Chan YC, Chung YC, Liao JW (2011). Food safety evaluation of papaya fruits resistant to papaya ring spot virus. J. Food Drug Anal. 19:269–280.

Yuet Ping, K., Darah, I., Yusuf, U. K., Yeng, C., & Sasidharan, S. (2012). Genotoxicity of Euphorbia hirta: an Allium cepa assay. Molecules, 17(7), 7782-7791.

Published

2022-03-20

How to Cite

KAYA, N. (2022). VIBURNUM OPULUS VE EQUISETUM ARVENSE EKSTRAKTI TİCARİ FORMÜLASYONLARININ ANTİ-SİTOTOKSİK VE ANTİ-GENOTOKSİK AÇIDAN DEĞERLENDİRİLMESİ. ICONTECH INTERNATIONAL JOURNAL, 6(1), 26-32. https://doi.org/10.46291/ICONTECHvol6iss1pp26-32

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